✦ LIBER ✦

Identification of drug metabolites in biological matrices by intelligent automated liquid chromatography/ tandem mass spectrometry

✍ Scribed by Linda L. Lopez; Xiao Yu; Donghui Cui; Margaret R. Davis

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 102 KB
Volume: 12
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19981130)12:22<1756::aid-rcm381>3.0.co;2-c

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid and systematic strategy for the identification of drug metabolites in biological matrices based on liquid chromatography -tandem mass spectrometry (LC/MS/MS) techniques was utilized for the identification of drug metabolites of the HIV protease inhibitor Indinavir. This strategy integrates intelligent realtime mass spectrometry with HPLC detection and a predictive strategy for detecting metabolites arising from common biotransformations, to rapidly elucidate structures of drug metabolites. Structures of metabolites generated from in vitro incubation mixtures of Indinavir were characterized from a single chromatographic analysis using the automated LC/MS/MS methodology, thus reducing data acquisition time and improving efficiency.

📜 SIMILAR VOLUMES

Characterization of metabolites of Celec

Characterization of metabolites of Celecoxib in rabbits by liquid chromatography/tandem mass spectrometry

✍ Ji Y. Zhang; YueFen Wang; Caroline Dudkowski; Dai-chang Yang; Ming Chang; Jinhua 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 191 KB 👁 2 views

The metabolism of the anti-inflammatory drug Celecoxib in rabbits was characterized using liquid chromatography (LC)/tandem mass spectrometry (MS/MS) with precursor ion and constant neutral loss scans followed by product ion scans. After separation by on-line liquid chromatography, the crude urine s

Automated identification of isotopically

Automated identification of isotopically labeled pesticides and metabolites by intelligent ‘real time’ liquid chromatography tandem mass spectrometry using a bench-top ion trap mass spectrometer

✍ Dieter M. Drexler; Philip R. Tiller; Sibylle M. Wilbert; Frederick Q. Bramble; J 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 229 KB 👁 1 views

A screening method was developed to specifically detect and identify isotopically labeled compounds automatically in real-time using a HPLC/ion trap mass spectrometer utilizing newly developed isotopic pattern recognition software. The ion trap mass spectrometer specifically detected isotopically la

Determination of the enantiomers of salb

Determination of the enantiomers of salbutamol and its 4-O-sulphate metabolites in biological matrices by chiral liquid chromatography tandem mass spectrometry

✍ Karina B. Joyce; Anne E. Jones; Rebecca J. Scott; Robert A. Biddlecombe; Stephen 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 246 KB 👁 1 views

Sensitive, mass spectrometry based bioanalytical methods are described for the determination of the R-and S-enantiomers of the b-agonist salbutamol (albuterol) and its 4-O-sulphate metabolite in human plasma and urine. In both methods samples are prepared by 96 well format solid phase extraction usi

Direct identification of yessotoxin in s

Direct identification of yessotoxin in shellfish by liquid chromatography coupled with mass spectrometry and tandem mass spectrometry

✍ Rosa Draisci; Luigi Giannetti; Luca Lucentini; Emanuele Ferretti; Luca Palleschi 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 145 KB 👁 2 views

A new method for the direct identification of yessotoxin (YTX), a polyether compound belonging to the diarrhoeic shellfish poisoning (DSP) toxins, using liquid chromatography coupled with mass spectrometry and tandem mass spectrometry (LC/MS and LC/MS/MS) is reported. Full-scan ionspray mass spectra

Rapid identification of saponins in plan

Rapid identification of saponins in plant extracts by electrospray ionization multi-stage tandem mass spectrometry and liquid chromatography/tandem mass spectrometry

✍ Meng Cui; Fengrui Song; Yu Zhou; Zhiqiang Liu; Shuying Liu 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 127 KB 👁 2 views

Electrospray ionization multi-stage tandem mass spectrometry (ESI-MS(n)) and liquid chromatography coupled with on-line mass spectrometry (LC/MS/MS) were applied to characterize saponins in crude extracts from Panax ginseng. The MS(n) data of the [M - H](-) ions of saponins can provide structural in

Use of monolithic silica columns to incr

Use of monolithic silica columns to increase analytical throughput for metabolite identification by liquid chromatography/tandem mass spectrometry

✍ Gordon Dear; Robert Plumb; Dave Mallett 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 129 KB