✦ LIBER ✦

Rapid identification of saponins in plant extracts by electrospray ionization multi-stage tandem mass spectrometry and liquid chromatography/tandem mass spectrometry

✍ Scribed by Meng Cui; Fengrui Song; Yu Zhou; Zhiqiang Liu; Shuying Liu

Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 127 KB
Volume: 14
Category: Article
ISSN: 0951-4198
DOI: 10.1002/1097-0231(20000730)14:14<1280::aid-rcm26>3.0.co;2-c

No coin nor oath required. For personal study only.

✦ Synopsis

Electrospray ionization multi-stage tandem mass spectrometry (ESI-MS(n)) and liquid chromatography coupled with on-line mass spectrometry (LC/MS/MS) were applied to characterize saponins in crude extracts from Panax ginseng. The MS(n) data of the M - H ions of saponins can provide structural information on the sugar sequences of the saccharide chains and on the sapogins of saponins. By ESI-MS(n), non-isomeric saponins and isomeric saponins with different aglycones can be determined rapidly in plant extracts. LC/MS/MS is a good complementary analytical tool for determination of isomeric saponins. These approaches constitute powerful analytical tools for rapid screening and structural assignment of saponins in plant extracts.

📜 SIMILAR VOLUMES

Analysis of Phosphorylated Terpenes by E

Analysis of Phosphorylated Terpenes by Electrospray Ionization Liquid Chromatography–Tandem Mass Spectrometry

✍ Iris Ney; Elke Jäger; Markus Herderich; Peter Schreier; Wilfried Schwab 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 362 KB 👁 2 views

Linalool, nerol, geraniol, citronellol and a-terpineol were phosphorylated and the products purified by adsorption chromatography on XAD-2. High performance liquid chromatography-tandem mass spectrometry (LC-MSMS) analysis using the electrospray ionization technique (ESI) revealed the occurrence of

Analysis of phytochelatin-cadmium comple

Analysis of phytochelatin-cadmium complexes from plant tissue culture using nano-electrospray ionization tandem mass spectrometry and capillary liquid chromatography/electrospray ionization tandem mass spectrometry

✍ Yen, Ten-Yang; Villa, Joseph A.; DeWitt, Jane G. 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 182 KB 👁 1 views

Phytochelatins (PCs, also known as class III metallothioneins), a family of sulfhydryl-rich peptides with the formula (g-GluCys) n Gly(Pc n , n = 2-11), are induced in plants, yeast and fungi exposed to heavy metals, and are thought to detoxify metals by forming PC-metal complexes. Although PCs have

Electrospray ionization mass spectrometr

Electrospray ionization mass spectrometry of 5-methyl-2′-deoxycytidine and its determination in urine by liquid chromatography/electrospray ionization tandem mass spectrometry

✍ Carlo Giorgio Zambonin; Francesco Palmisano 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 79 KB 👁 2 views

The behaviour of 5-methyl-2'-deoxycytidine (m 5 dCyd, claimed to be a potential marker for leukaemia) during the electrospray process was studied. In particular, considerations concerning the effect of solution chemistry (e.g. analyte concentration, pH, etc.) on electrospray ionization mass spectra

Direct identification of yessotoxin in s

Direct identification of yessotoxin in shellfish by liquid chromatography coupled with mass spectrometry and tandem mass spectrometry

✍ Rosa Draisci; Luigi Giannetti; Luca Lucentini; Emanuele Ferretti; Luca Palleschi 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 145 KB 👁 1 views

A new method for the direct identification of yessotoxin (YTX), a polyether compound belonging to the diarrhoeic shellfish poisoning (DSP) toxins, using liquid chromatography coupled with mass spectrometry and tandem mass spectrometry (LC/MS and LC/MS/MS) is reported. Full-scan ionspray mass spectra

Rapid characterization of urinary metabo

Rapid characterization of urinary metabolites of pibutidine hydrochloride in humans by liquid chromatography/electrospray ionization tandem mass spectrometry

✍ K. Kato; S. Jingu; N. Ogawa; S. Higuchi 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 108 KB 👁 1 views

The metabolic products of pibutidine hydrochloride, a new H 2 -receptor antagonist, in human urine after oral administration of 40 mg/man were characterized by high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS) with electrospray ionization (ESI). Two-stage collision-induced d

Quantitative determination of ochratoxin

Quantitative determination of ochratoxin A by liquid chromatography/electrospray tandem mass spectrometry

✍ Lau, B. P.-Y.; Scott, P. M.; Lewis, D. A.; Kanhere, S. R. 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 163 KB 👁 1 views

Mass spectrometry of ochratoxin A (OTA) and B (OTB) under electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) was studied. ESI offers higher sensitivities and less fragmentation than APCI. A sensitive LC/MS/MS method for the determination of ochratoxin A (OTA) in human