Identification of lignans by liquid chromatography-electrospray ionization ion-trap mass spectrometry

✍ Scribed by Patrik C. Eklund; M. Josefin Backman; Leif Å. Kronberg; Annika I. Smeds; Rainer E. Sjöholm

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 314 KB
• Volume: 43
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/jms.1276

✦ Synopsis

Abstract

The fragmentation pattern of 30 compounds belonging to different classes of the lignan family was studied by liquid chromatography‐electrospray ionization ion‐trap mass spectrometry. On the basis of the observed fragmentation patterns, identification of different types of lignans was achieved. For example, dibenzylbutyrolactone lignans showed a characteristic fragmentation pathway by the loss of 44 Da (CO~2~) from the lactone moiety, whereas dibenzylbutanediols showed a loss of 48 Da by a combined loss of formaldehyde and water from the 1,4‐butanediol moiety. Lignan glycosides readily lost the sugar residue to give the parent lignan as their primary product ion. In addition, several compound‐specific fragmentations were observed and used for identification of individual compounds.

A versatile method for analyses of lignans was developed using LC separation on a C8 column followed by fragmentation and detection of ions produced in the ion trap. Copyright © 2007 John Wiley & Sons, Ltd.