A method combining flow sorting and molecular cytogenetic techniques for the identification of unknown marker chrornosomes is described. In this study, the bladder tumor cell line 182 was used, which was known t o carry a marker chromosome of the size of chromosome 7 in every cell. From the cytogene
Identification of flow-sorted chromosomes by G-banding and in situ hybridization
β Scribed by Dipl. Biol. B. Rommel; K.-J. Hutter; J. Bullerdiek; S. Bartnitzke; K. Goerttler; W. Schloot
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 778 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Four patients with acute leukemia are presented in whom conventional karyotypic analysis had revealed complex numerical and structural changes including unidentifiable (marker) chromosomes. It is demonstrated that the application of fluorescence in situ hybridization (FISH) may contribute to the ide
Alterations of the distal portion of chromosome I p are a recurrent abnormality of several types of human cancer. In this study we show that chromosomal in situ suppression hybridization with a regional lp36 D N A bank generated by microdissection and microcloning can be employed to detect transloca
## Abstract Double minute chromosomes (dmin) are small chromatin bodies consisting of genes amplified in an extrachromosomal location. dmins are uncommon in hematologic malignancies; they are seen primarily in acute myeloid leukemia, with amplification of the __MYC__ oncogene or, less frequently, t