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Identification of a tumor marker chromosome by flow sorting, DNA amplification in vitro, and in situ hybridization of the amplified product

✍ Scribed by Gert A. Boschman; Charles H. C. M. Buys; Anneke Y. Van Der Veen; Wim Rens; Jan Osinga; Rosalyn M. Slater; Jacob A. Aten

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 605 KB
Volume: 6
Category: Article
ISSN: 1045-2257
DOI: 10.1002/gcc.2870060104

No coin nor oath required. For personal study only.

✦ Synopsis

A method combining flow sorting and molecular cytogenetic techniques for the identification of unknown marker chrornosomes is described. In this study, the bladder tumor cell line 182 was used, which was known t o carry a marker chromosome of the size of chromosome 7 in every cell. From the cytogenetic analysis of Q-banded rnetaphase cells, it was shown to be composed of -40% presumably the greater part of chromosome 20 and for the rest microscopically unidentifiable material.

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