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Identification of dimer impurities in ampicillin and amoxicillin by capillary LC and tandem mass spectrometry

✍ Scribed by Chi-Yu Lu; Chia-Hsien Feng

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
344 KB
Volume
30
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

A micro‐scale liquid chromatography electrospray ionization tandem mass spectrometric method was developed for the identification of polymerized impurities in ampicillin and amoxicillin in aqueous solution. Ampicillin and amoxicillin are broad‐spectrum antibiotics and widely used for the treatment of human and animal infections. In this study ampicillin, amoxicillin, and their dimers were trapped in a 5‐cm capillary column containing C~18~ sorbents. The analytes were separated on a reversed‐phase column and introduced into the mass spectrometer via a nanospray ion source. An isocratic mobile phase consisting of 1% formic acid‐acetonitrile (50 : 50, v/v) was used. For identification, the fragment ions of the analytes were monitored. The aim of the present study was to develop an optimized quality control method for the analysis of high molecular weight impurities of ampicillin and amoxicillin.

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