✦ LIBER ✦

Identification and Mapping of Phosphocholine in Animal Tissue by Static Secondary Ion Mass Spectrometry and Tandem Mass Spectrometry

✍ Scribed by J. M. McMahon; R. T. Short; C. A. McCandlish; J. T. Brenna; P. J. Todd

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 638 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199602)10:3<335::aid-rcm516>3.0.co;2-w

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✦ Synopsis

Secondary ion mass spectra and images were obtained from animal tissue samples using less than 10(13) primary ions/cm2. The mass spectra showed abundant peaks at m/z 184 and m/z 86. Tandem mass spectrometry (MS/MS) was used to identify the source of these ions as phosphocholine. Secondary ion images obtained using MS/MS were used to show that m/z 86 is an abundant gas-phase fragment ion derived from m/z 184. These results are discussed in terms of the physiology of the samples investigated.

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