We report the first direct method for the identification of the vitamin K-dependent Ca2+ binding amino acid, gamma-carboxyglutamic acid (Gla), in the sequencing of proteins. The carboxyl groups on the protein are first converted to methyl esters with methanolic HCl, a procedure that reduces the pola
Identification and isolation of vitamin K-dependent proteins by HPLC
β Scribed by Robert D. Litwiller; Richard J. Jenny; Kenneth G. Mann
- Book ID
- 107714367
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 1008 KB
- Volume
- 158
- Category
- Article
- ISSN
- 0003-2697
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There are seven known vitamin K-dependent proteins in blood. These proteins require calcium ion for expressing their full biological activities. Calcium ion also induces conformational changes in this class of proteins. Taking advantage of the ligand induced conformational changes, a number of uniqu
folgt anhand (tiefer und hoher) benachbartertaglich neu zu ermittelnder -Standardvergleichsextinktionen aus dem Bereich 50-300 IED,/ml Standard-MeBlosung und der Extinktion des Reagentienblindwertes. Die Wiederfindungsrate (Recovery) betrug 75.5 O h . 2. Bestimmung von Gesamt-, freiem und vereJterte