Hypoxanthine-guanine phosphoribosyltransferase in human erythroid cells: Properties of the isozymes
โ Scribed by Gerald G. Johnson; Sharon A. Nash
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 659 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0006-2928
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๐ SIMILAR VOLUMES
Male New Zealand White rabbits were immunized with human adenine phosphoribosyltransferase (APRT) and hypoxanthine-guanine phosphoribosyltransferase (HGPRT), which were purified about 2000-fold and 800-fold, respectively, from erythrocytes by DEAE-cellulose chromatography, ammonium sulfate precipita
The method of isoelectric focusing in polyacrylamide gel was used to separate HGPRT isoenzymes in crude hemolysates of human and rat erythroeytes. HGPRT from erythrocytes of a normal human male donor consistently revealed three peaks of activity. Their mean isoelectrie points, using pH 5-7 range amp