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Analysis of human hypoxanthine-guanine phosphoribosyl transferase isozymes by isoelectric focusing in polyacrylamide gel

✍ Scribed by Vazken M. Kaloustian; Zuhayr L. Awdeh; Ruwayda T. Hallal; Nabil W. Wakid

Publisher: Springer
Year: 1973
Tongue: English
Weight: 237 KB
Volume: 9
Category: Article
ISSN: 0006-2928
DOI: 10.1007/bf00485594

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✦ Synopsis

The method of isoelectric focusing in polyacrylamide gel was used to separate HGPRT isoenzymes in crude hemolysates of human and rat erythroeytes. HGPRT from erythrocytes of a normal human male donor consistently revealed three peaks of activity. Their mean isoelectrie points, using pH 5-7 range ampholytes, were, peak I, pI 6.00; peak II, pI 5.83; and peak III, pI 5.71. Peak Ill was wide and tailed. It always had a shoulder with a mean pI of 5.62. HGPRT from rat erythroeytes revealed two peaks of activity, corresponding to isoelectric points of 5.90 and 5.80. The method of isoeleetric focusing in polyacrylamide gel is presented as a new way of detecting isoenzyme patterns.

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