Microscale isoelectric focusing studies of mouse and human hypoxanthine-guanine phosphoribosyl transferases

✍ Scribed by Kenneth L. Roy; Frank H. Ruddle

• Publisher: Springer
• Year: 1973
• Tongue: English
• Weight: 559 KB
• Volume: 9
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00487447

✦ Synopsis

A mieroscale isoelectric focusing technique has been developed and used to study hypoxanthine-guanine phosphoribo~yl transferase (HGPRT," E.C. 2.4.2.8, inosinate-guanylate :pyrophosphate phosphoribosyl transferase) activities in mouse and human cell lines. The enzymes of both mouse and human origin are shown to exhibit considerable heterogeneity, but each type has a unique range of isoelectric pH. The enzyme of a mouse × human hybrid cell line, derived by fusion of HGPRT-parental cells, gives a homogeneous peak of activity, unlike the wild-type enzyme of either parent. The possibiBty is suggested that this enzyme activity is due to intra-allelie eomplementation.