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Analysis of human erythrocyte glucose 6-phosphate dehydrogenase isozymes by isoelectric focusing in polyacrylamide gel

✍ Scribed by Vazken M. Kaloustian; Shirine H. Idriss-Daouk; Ruwayda T. Hallal; Z. L. Awdeh

Publisher: Springer
Year: 1974
Tongue: English
Weight: 427 KB
Volume: 12
Category: Article
ISSN: 0006-2928
DOI: 10.1007/bf00487527

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✦ Synopsis

The method of isoeIectric focusing in polyacrylamide gel was used to separate G6PD isozymes in crude hemolysates of human, rabbit, and rat erythroeytes. G6PD (B) from erythrocytes of a normal human male donor revealed six bands of activity. Their mean isoelectric points, using pH 3-10 and 5-8 range empholytes, were pI 7.04for band I, pI 6.60for band II, pI 6.37for band Ill, pI 6.11 for band IV, pI 5.94for band V, pI 5.79for band VI. G6PD from rabbit and rat erythroeytes revealed completely different multiple band patterns. The method of isoelectric focusing in polyacrylamide gel is presented as a new way of detecting G6PD isozyme patterns.

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