Assay of hypoxanthine-guanine phosphoribosyltransferase in human fibroblast lysates: Inactivation of nucleotidase
โ Scribed by Gerald G. Johnson; John W. Littlefield
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 569 KB
- Volume
- 92
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The X-linked hypoxanthine-guanine phosphoribosyl-respectively. These data were similar to the reported transferase (hprt) gene is a target of analyses of in values. The mean MFs in the nine colon cancer pavivo mutation frequencies in circulating T-lympho-tients (10.6 { 7.3 1 10 06 ) were not signifi
A micromodification of the method of HGPRT and APRT assay is described, which measures the incorporation of 14C hypoxanthine and 14C adenine into cultured skin fibroblasts and amniotic cells grown on microtiter plates. Only about 10000 cells are needed per assay. By this method HGPRT deficient cells
Mutations in the X-linked hypoxanthine-guanine phosphoribosyl transferase gene (HPRT) result in deficiencies of HPRT enzyme activity, which may cause either a severe form of gout or Lesch-Nyhan syndrome depending on the residual enzyme activity. Mutations leading to these diseases are heterogeneous