Hyphenated techniques for the analysis of heparin and heparan sulfate
โ Scribed by Bo Yang; Kemal Solakyildirim; Yuqing Chang; Robert J. Linhardt
- Publisher
- Springer
- Year
- 2010
- Tongue
- English
- Weight
- 841 KB
- Volume
- 399
- Category
- Article
- ISSN
- 1618-2650
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๐ SIMILAR VOLUMES
A modification of a method for hexosamine analysis is presented which adapts it to measurement of sulfaminohexose in heparins and heparan sulfates. Unlike methods of sulfaminohexose analysis based upon coupling with indole, the absorptivity of polymeric and monomeric hexosamines is identical. N-Sulf
has been established for the determination of uronate residues in glycosaminoglycans (GAGs) such as dermatan sulfate (DS), heparin (HP), and heparan sulfate (HS). Because of variation in the sulfonation positions in DS, HP, or HS, interpretation of spectra is difficult. Solvolysis was applied to rem
Heparin stimulates 2-3-fold, in a concentration-dependent manner, the synthesis of heparan sulfate secreted by cultured endothelial cells. The increase in synthetic rate takes place immediately after exposure of the cells to heparin, affects only heparan sulfate, and i s specific for the endothelial