A rapid, sensitive and accurate high-performance capillary electrophoresis method is described for the determination of the sulfation pattern of heparin and heparan sulfate disaccharides. The analysis, performed after enzymic degradation of the polysaccharides with heparinase and heparinases II and
Disaccharide compositional analysis of heparin and heparan sulfate using capillary zone electrophoresis
β Scribed by S.A. Ampofo; H.M. Wang; R.J. Linhardt
- Book ID
- 107714955
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 711 KB
- Volume
- 199
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
High-voltage capillary zone electrophoresis (CZE) has been used for the first time in the analysis of non-, mono-, di-, and trisulfated disaccharides derived from chondroitin sulfate, dermatan sulfate, and hyaluronic acid. These glycosaminoglycans are first depolymerized using polysaccharide lyases.
We have developed techniques for the separation of unsulfated (2-acetamido-2-deoxy-3-O-(4-deoxy-alpha-L-threo- hex-4-enopyranosyluronicacid)-D-galactose and -D-glucose), monosulfated (2-acetamido-2-deoxy-3- O-(4-deoxy-2-O-sulfo-alpha-L-threo-hex-4-enopyranosyluronic acid)-D-galactose and 2-acetamido