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An accurate colorimetric method for measurement of sulfaminohexose in heparins and heparan sulfates

✍ Scribed by Robert E. Hurst; Juanita M. Settine

Publisher: Elsevier Science
Year: 1981
Tongue: English
Weight: 349 KB
Volume: 115
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(81)90528-5

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✦ Synopsis

A modification of a method for hexosamine analysis is presented which adapts it to measurement of sulfaminohexose in heparins and heparan sulfates. Unlike methods of sulfaminohexose analysis based upon coupling with indole, the absorptivity of polymeric and monomeric hexosamines is identical. N-Sulfated hexosamines are specifically deaminated in 33% acetic acid to yield free 2,Sanhydromannose residues which are then coupled to the color reagent 3-methyl-2-benzothiazolinone hydrazone hydrochloride. The sulfaminohexose content of a variety of heparins and heparan sulfates was determined with this methodology and compared with the indole-coupling method. Interferences by amino acids, proteins, and neutral sugar were evaluated in the sulfaminohexose assay and in the originally reported procedure fo; total hexosamine analysis.

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