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Heparin stimulates the synthesis and modifies the sulfation pattern of heparan sulfate proteoglycan from endothelial cells

✍ Scribed by Helena B. Nader; Vincenzo Buonassisi; Patricia Colburn; Carl P. Dietrich

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
605 KB
Volume
140
Category
Article
ISSN
0021-9541

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✦ Synopsis

Heparin stimulates 2-3-fold, in a concentration-dependent manner, the synthesis of heparan sulfate secreted by cultured endothelial cells. The increase in synthetic rate takes place immediately after exposure of the cells to heparin, affects only heparan sulfate, and i s specific for the endothelial cell. No stimulation by other glycosaminoglycans was observed. Analysis of the disaccharide products formed by the action of heparitinases reveals a higher degree of sulfation of the uronic acid residues in the heparan sulfate of cells exposed to heparin.

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