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Hydrolysis of poly-L-lysine by pronase

✍ Scribed by R. Sass; W. Thiemann


Publisher
Wiley (John Wiley & Sons)
Year
1973
Tongue
English
Weight
219 KB
Volume
12
Category
Article
ISSN
0006-3525

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✦ Synopsis


Abstract

Pronase E is able to hydrolyze poly‐L‐lysine in 100% yield into the monomer L‐lysine. The experiments show an intermediate production of tri‐L‐lysine and di‐L‐lysine that are clipped off from the polymeric peptide. During the hydrolytic process a number of characteristic amino acids are released from the enzyme that may indicate its activation. Amino acids as well as mono‐L‐lysine are decomposed by pronase E after a period of several days.


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