The hydrolysis of poly (L-prolyl-glycyl-L-prolyl) by bacterial collagenase

✍ Scribed by Elvin Harper; Arieh Berger; Ephraim Katchalski

• Publisher: Wiley (John Wiley & Sons)
• Year: 1972
• Tongue: English
• Weight: 337 KB
• Volume: 11
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1972.360110807

✦ Synopsis

Abstract

Poly (L‐Prolyl‐Glycyl‐L‐Prolyl), a polymer which resembles collagen by physical and immunochemical criteria, has been shown to serve as a substrate for the highly specific bacterial collagenase obtained from Clostridium histolyticum. The postulated reaction products Gly Pro, Gly Pro Pro, and Pro Gly Pro Pro have been isolated. The enzyme has been employed as an analytical tool in elucidating the sequence of synthesized polymers of proline and glycine.