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Conformational change of poly-Nε-glutary-L-lysine and poly-Nε-succinyl-L-lysine in aqueous salt solutions

✍ Scribed by Norio Murai; Shintaro Sugai

Publisher
Wiley (John Wiley & Sons)
Year
1974
Tongue
English
Weight
464 KB
Volume
13
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The conformational changes of poly‐N^ε^‐glutaryl‐L‐lysine (PGL) and poly‐N^ε^‐succinyl‐L‐lysine (PSL) in various salt solutions were studied by use of ORD and potentiometric titration measurements. The addition of alkali metal salts to the fully ionized PGL or PSL solution caused helix formation. The helical content of the polymers increases in the following sequences: at salt concentration 0–2 M, CsCl < KCl < LiCl < NaCl; and at 2–3 M, LiCl < CsCl < KCl ∼ NaCl. The preferential binding of the solvent components with various alkali metal salts of PGL or PSL was measured in LiCl, NaCl, and KCl solutions by means of equilibrium dialysis and differential refractometry. It was found that with increasing salt concentration, the polymers were preferentially hydrated in NaCl and KCl soultions; however the salt was preferentially bound to the polymers in LiCl solution. Such preferential binding was suggested to be closely related to conformational change. The addition of CaCl~2~ to polymer solutions led to the stabilization of the helical structure of PGL or PSL.

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