The chemiluminescent emission from the luminoI-HaOa-horseradish peroxidase system was enhanced between 2 and 80 times, during the first 5 min, by chemical indicators, such as phenolphthalein, cresolphthalein, phenol red, cresol red, benzidine and o-tolidine. Phenol derivatives showed emission maxima
Hydrogen peroxide assay by using enhanced chemiluminescence of the luminol-H2O2-horseradish peroxidase system: Comparative studies
✍ Scribed by A.Navas Díaz; F.García Sanchez; J.A.González García
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 379 KB
- Volume
- 327
- Category
- Article
- ISSN
- 0003-2670
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The chemiluminescence of the luminol-H 2 O 2 -horseradish peroxidase system is increased by fluorescein. Fluorescein produces an enhancement of the luminol chemiluminescence similar to that of phenolphthalein, by an energy transfer process from luminol to fluorescein. The maximum intesity and the to
2-Naphthyl acetate acts as a pro-enhancer of the Iuminol-H2O2-horseradish peroxidase reaction. Cholinesterase hydrolyses the bound acetyl group and produces 2naphthol, and this compound is an enhancer of the chemiluminescent reaction. We studied the kinetics of chemiluminescent emission and the infl