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Chemical indicators as enhancers of the chemiluminescent luminol-H2O2-horseradish peroxidase reaction

✍ Scribed by A.Navas Díaz; F. García Sánchez; J.A. González García

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
615 KB
Volume
87
Category
Article
ISSN
1010-6030

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✦ Synopsis

The chemiluminescent emission from the luminoI-HaOa-horseradish peroxidase system was enhanced between 2 and 80 times, during the first 5 min, by chemical indicators, such as phenolphthalein, cresolphthalein, phenol red, cresol red, benzidine and o-tolidine. Phenol derivatives showed emission maxima at pH 8.5, while aniline derivatives showed emission maxima at pH 10.5. The enhancers with a methyl group at the ortho or para position to the OH and NH2 groups in the benzene ring showed an inhibitory effect when their concentrations were increased.

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