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Enhancer effect of fluorescein on the luminol-H2O2-horseradish peroxidase chemiluminescence: energy transfer process

✍ Scribed by Navas Díaz, A. ;González García, J. A. ;Lovillo, J.

Publisher
John Wiley and Sons
Year
1997
Weight
135 KB
Volume
12
Category
Article
ISSN
0884-3996

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✦ Synopsis

The chemiluminescence of the luminol-H 2 O 2 -horseradish peroxidase system is increased by fluorescein. Fluorescein produces an enhancement of the luminol chemiluminescence similar to that of phenolphthalein, by an energy transfer process from luminol to fluorescein. The maximum intesity and the total chemiluminescence emission (between 380 and 580 nm) of luminol with fluorescein was more than three times greater than without fluorescein; however, the emission duration was shorter. The emission spectra in the presence of fluorescein had two maxima (425 and 535 nm) and the enhancement was dependent on pH and fluorescein concentration. A mechanism is proposed to explain these effects.

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✍ Díaz, A. Navas ;Sánchez, F. García ;González Garcia, J. A. 📂 Article 📅 1998 🏛 John Wiley and Sons ⚖ 159 KB 👁 1 views

Systematic studies on phenol derivatives facilitates an explanation of the enhancement or inhibition of the luminol-H2O2-horseradish peroxidase system chemiluminescence. Factors that govern the enhancement are the one-electron reduction potentials of the phenoxy radicals (PhO./PhOH) vs. luminol radi