The chemiluminescent emission from the luminoI-HaOa-horseradish peroxidase system was enhanced between 2 and 80 times, during the first 5 min, by chemical indicators, such as phenolphthalein, cresolphthalein, phenol red, cresol red, benzidine and o-tolidine. Phenol derivatives showed emission maxima
Thin-layer chromatography with chemiluminescent detection of enhancers of the luminol-H2O2-peroxidase system
✍ Scribed by A. Navas Díaz; F. García Sánchez; J.A. González García
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 332 KB
- Volume
- 724
- Category
- Article
- ISSN
- 1873-3778
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Phenols which markedly enhance chemiluminescence in the horseradish peroxidase catalysed oxidation of luminol by hydrogen peroxide show anomalously high reactivity (by factors of -102 compared with published Hammett correlations) in the reduction of the enzyme intermediates, Compound I and Compound
Systematic studies on phenol derivatives facilitates an explanation of the enhancement or inhibition of the luminol-H2O2-horseradish peroxidase system chemiluminescence. Factors that govern the enhancement are the one-electron reduction potentials of the phenoxy radicals (PhO./PhOH) vs. luminol radi