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Assay of cholinesterase using a proenhancer of the luminol-H2O2-horseradish peroxidase reaction

✍ Scribed by Díaz, A. Navas ;Sanchez, F. García ;García, J. A. González ;Del Rio, V. Bracho

Publisher
John Wiley and Sons
Year
1995
Weight
395 KB
Volume
10
Category
Article
ISSN
0884-3996

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✦ Synopsis

2-Naphthyl acetate acts as a pro-enhancer of the Iuminol-H2O2-horseradish peroxidase reaction. Cholinesterase hydrolyses the bound acetyl group and produces 2naphthol, and this compound is an enhancer of the chemiluminescent reaction. We studied the kinetics of chemiluminescent emission and the influence of 2-naphthyl acetate and cholinesterase enzyme concentrations. The cholinesterase concentration versus chemiluminescence intensity maximum was linear for cholinesterase between 0 and 181 pU/mL, with a detection limit of 8pU/mL and a relative standard deviation of 9.5% ( n = 3). for a sample containing 90.67pU/mL of cholinesterase

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