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Human mutant cell lines with altered RNA polymerase II

✍ Scribed by Monica T. M. Shander; Carlo Croce; Roberto Weinmann

Book ID
102882268
Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
637 KB
Volume
113
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

A human fibrosarcoma cell line, HT‐1080‐6TG‐9AM, resistant to α‐amanitin at concentrations up to 10 μg/ml, was isolated after ethylmethanesulfonate mutagenesis and stepwise selection. The mutation is stable and dominant. RNA polymerase II purified from the mutant cells showed an altered affinity for labeled α‐amanitin and the sensitivity of the enzyme to the fungal toxin was decreased 50‐to 100‐fold. This functional test demonstrated that the biochemical basis for the resistance of the cells to α‐amanitin is due to an alteration of RNA polymerase II.

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