A structural gene for sigma factor (rpoD) of DNA-dependent RNA polymerase (RNA nucleotidyltransferase; nucleoside-triphosphate: RNA nucleotidyltransferase, E.C. 2.7.7.6) was mapped precisely by a set of F' factors including those already published (Proc. Natl. Acad. Sci. USA. 74, 1831-1835 (1977)).
Cold sensitive mutant of Escherichia coli with altered RNA polymerase
β Scribed by Nikiforov, V. G. ;Kalyaeva, E. S. ;Velkov, V. V.
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 357 KB
- Volume
- 130
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Temperature-sensitive mutants of Escherichia coli that are unable to grow at high temperature can be obtained among those selected for resistance to streptovaricin or rifampicin at low temperature (Yura et al., 1973). One of these mutants (KY5323) that was supposed to carry a single mutation affecti
A cold-sensitive mutation in the rpoB gene for the RNA polymerase beta subunit increasing the temperature of promoter opening on T2 phage DNA was obtained in Escherichia coli. The mutation also affects the stages preceding promoter opening by increasing the dissociation rate of RNA polymerase--DNA c
Starting with an E. coli merodiploid strain (rpoB + (rifS)/rpoB-(rifR)) containing equal amounts of rifampicin-sensitive and resistant RNA polymerase activities, mutants were isolated that had a disproportionately high ratio of the rifampicin-resistant enzyme activityβ’ With one strain it is shown th