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RNA polymerase mutants of Escherichia coli

โœ Scribed by Iwakura, Y. ;Ishihama, A. ;Yura, T.

Publisher
Springer
Year
1973
Tongue
English
Weight
846 KB
Volume
121
Category
Article
ISSN
0026-8925

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Temperature-sensitive mutants of Escherichia coli that are unable to grow at high temperature can be obtained among those selected for resistance to streptovaricin or rifampicin at low temperature (Yura et al., 1973). One of these mutants (KY5323) that was supposed to carry a single mutation affecti

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A mutant of Escherichia coli K12 is described in which sigma and alpha subunits of the DNA-dependent RNA polymerase (EC 2.7.7.6) are produced at the rates much higher than in the normal strain. The rate of synthesis for sigma subunit was found to be at least 10-times higher, though the rapid degrada