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Escherichia coli merodiploids with altered ratios of episomal to chromosomal RNA polymerase activity

✍ Scribed by Tittawella, Ivor P. B.

Publisher: Springer
Year: 1981
Tongue: English
Weight: 440 KB
Volume: 184
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00352530

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✦ Synopsis

Starting with an E. coli merodiploid strain (rpoB + (rifS)/rpoB-(rifR)) containing equal amounts of rifampicin-sensitive and resistant RNA polymerase activities, mutants were isolated that had a disproportionately high ratio of the rifampicin-resistant enzyme activity• With one strain it is shown that the mutation responsible for this phenotype is closely linked to the rifR (rpoB-) allele.

The enzyme RNA polymerase is responsible for most if not all RNA synthesis in E. coli. The RNA polymerase subunits e, ~, /~', and ~r, which occur in the stoichiometry ~2flfl'ff in the complete enzyme (holoenzyme) are encoded in the genes rpoA (72 rain on the chromosome), rpoB (89.5 min), rpoC (89.5 rain), and rpoD (66 rain), respectively (Scaife 1976;Lathe 1978;Yura and Ishihama 1979;Bachmann and Low 1980). rpoB and rpoC share an operon with the ribosomal protein genes rplJ and rplL,

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