𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Human herpesvirus 6 infection of human epidermal cell line: Pathogenesis of skin manifestations

✍ Scribed by Dr. Tetsushi Yoshikawa; Fumi Goshima; Shiho Akimoto; Takao Ozaki; Takuya Iwasaki; Takeshi Kurata; Yoshizo Asano; Yukihiro Nishiyama

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
185 KB
Volume
71
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV‐6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV‐6. HHV‐6 causes abortive infection in the A431 cells, because neither late antigen (OHV‐3 antigen) nor progeny virus is produced. Maximum levels of HHV‐6 antigen (IEA/ex3 antigen)‐positive cells (36.4%) were observed 48 hr after viral infection. Cocultivation of HHV‐6‐infected cord blood mononuclear cells with A431 cells was necessary for the establishment of a sufficient level of viral infection. Cell‐to‐cell contact between the infected cord blood mononuclear cells and A431 cells was crucial for increasing infection efficiency. To determine the biological effect of HHV‐6 infection, flow cytometric analysis was carried out in HHV‐6‐ and mock‐infected A431 cells. Although no alteration was observed in VCAM‐1 and ELAM‐1 expression, that of HLA‐ABC, HLA‐DR, and ICAM‐1 was upregulated after infection with HHV‐6. J. Med. Virol. 71:62–68, 2003. © 2003 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Reactivation of human herpesvirus 6 by i
Reactivation of human herpesvirus 6 by infection of human herpesvirus 7
✍ Tanaka-Taya, Keiko; Kondo, Toshio; Nakagawa, Naoko; Inagi, Reiko; Miyoshi, Hirok 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 375 KB 👁 1 views

We have attempted to reactivate human herpesvirus 6 (HHV-6) by infection with HHV-7 using childhood exanthem subitum patients in vitro. Peripheral blood mononuclear cells (PBMCs) were collected from children who had a history of exanthem subitum(ES) by HHV-6 and were infected by human herpesvirus 7

Infection of a human retinal pigment epi
Infection of a human retinal pigment epithelial cell line with human herpesvirus 6 variant A
✍ Arao, Yujiro; Soushi, Sumi; Sato, Yuko; Moriishi, Eiko; Ando, Yasutaka; Yamada, 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 525 KB

A retinal pigment epithelial (RPE) cell line (K-1034) was examined for its susceptibility to human herpesvirus 6 variant A (HHV-6A). Exposure of K-1034 cells to HHV-6A induced the formation of multinucleated giant cells, which was suppressed by an inhibitor of viral DNA synthesis. In the giant cells

Latent infection of human herpesvirus 6
Latent infection of human herpesvirus 6 in astrocytoma cell line and alteration of cytokine synthesis
✍ Tetsushi Yoshikawa; Yoshizo Asano; Shiho Akimoto; Takao Ozaki; Takuya Iwasaki; T 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 197 KB

## Abstract In order to study the pathogenesis of HHV‐6 infection in central nervous system disorders, U251 cell line was infected with freshly isolated variant B HHV‐6. Although IEA/ex 3 antigen (immediate early protein) was detected in infected cell nuclei, neither the presence of OHV‐3 antigen (

Quantitative analysis of human herpesvir
Quantitative analysis of human herpesvirus 6 cell tropism
✍ L. De Bolle; J. Van Loon; E. De Clercq; Lieve Naesens 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 400 KB

## Abstract Although HHV‐6A and ‐6B are known to replicate preferably in human T‐lymphocytes, in vitro infection of several other cell types has been described. Also, the finding that both variants use the ubiquitous molecule CD46 as a membrane receptor fully supports the possibility of a broad cel

Interaction of human herpesvirus 6 with
Interaction of human herpesvirus 6 with human CD34 positive cells
✍ Hiroki Isomura; Mariko Yoshida; Hikaru Namba; Masao Yamada 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 254 KB

## Abstract We reported previously that human herpesvirus 6 (HHV‐6) suppresses hematopoietic colony formation of erythroid (BFU‐E), granulocyte/macrophage (CFU‐GM), and megakaryocyte (CFU‐Meg) lineages in vitro. Here we describe the interaction between HHV‐6 and human CD34+ cells, which are a major

Evaluation of active human herpesvirus 6
Evaluation of active human herpesvirus 6 infection by reverse transcription-PCR
✍ Tetsushi Yoshikawa; Shiho Akimoto; Naoko Nishimura; Takao Ozaki; Masaru Ihira; M 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 72 KB

## Abstract Monitoring of active human herpesvirus 6 (HHV‐6) infection is important for distinguishing between reactivation and latency of the virus. The reverse transcription polymerase chain reaction (RT‐PCR) may be a useful tool in order to distinguish active and latent HHV‐6 infection. An RT‐PC