Reactivation of human herpesvirus 6 by infection of human herpesvirus 7

✍ Scribed by Tanaka-Taya, Keiko; Kondo, Toshio; Nakagawa, Naoko; Inagi, Reiko; Miyoshi, Hiroko; Sunagawa, Tomimasa; Okada, Shintaro; Yamanishi, Koichi

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 375 KB
• Volume: 60
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/(sici)1096-9071(200003)60:3<284::aid-jmv6>3.0.co;2-8

✦ Synopsis

We have attempted to reactivate human herpesvirus 6 (HHV-6) by infection with HHV-7 using childhood exanthem subitum patients in vitro. Peripheral blood mononuclear cells (PBMCs) were collected from children who had a history of exanthem subitum(ES) by HHV-6 and were infected by human herpesvirus 7 (HHV-7) in vitro. The antigen positive rate to HHV-6 started to increase 7 days after the infection and reached a maximum by Day 15 using an immunofluorescence antibody test. The copy number of HHV-6 DNA also increased in the samples in 10 days after infection in vitro. No antigen or increase in DNA was detected in PBMCs, that were mock-infected or infected with supernatant of stock virus after ultracentrifugation, suggesting that an infection by HHV-7 is necessary to reactivate HHV-6. In the paired sera samples during the acute and the convalescent phases of ES, seven to ten bands, that were specific for HHV-6, were recognized in samples from the acute phase, and at least 5 dominant polypeptides were found more intensively after HHV-7 infection.