𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Interaction of human herpesvirus 6 with human CD34 positive cells

✍ Scribed by Hiroki Isomura; Mariko Yoshida; Hikaru Namba; Masao Yamada

Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
254 KB
Volume
70
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We reported previously that human herpesvirus 6 (HHV‐6) suppresses hematopoietic colony formation of erythroid (BFU‐E), granulocyte/macrophage (CFU‐GM), and megakaryocyte (CFU‐Meg) lineages in vitro. Here we describe the interaction between HHV‐6 and human CD34+ cells, which are a major source of hematopoietic progenitor cells. CD34+ cells were immunomagnetically isolated from cord blood mononuclear cells using anti‐CD34+ antibodies coated onto either Dynabeads™ or MACS beads. The CD34+ population selected with Dynabeads showed a broad range of fluorescence. The population selected with MACS beads showed a narrow range of fluorescence. After infection with HHV‐6, two transcripts of the immediate early genes were detected with both cell populations. HHV‐6 suppressed colony formation of BFU‐E, CFU‐GM, and CFU‐Meg. HHV‐6 suppressed cell growth after 3 to 7 days culture in the presence of thrombopoietin (TPO). More differentiated CD34+ cells were more susceptible to the effects of HHV‐6. These data indicate that the targets for hematopoietic suppression by HHV‐6 are the differentiated cells. J. Med. Virol. 70:444–450, 2003. © 2003 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Quantitative analysis of human herpesvir
Quantitative analysis of human herpesvirus 6 cell tropism
✍ L. De Bolle; J. Van Loon; E. De Clercq; Lieve Naesens 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 400 KB

## Abstract Although HHV‐6A and ‐6B are known to replicate preferably in human T‐lymphocytes, in vitro infection of several other cell types has been described. Also, the finding that both variants use the ubiquitous molecule CD46 as a membrane receptor fully supports the possibility of a broad cel

Infection of a human retinal pigment epi
Infection of a human retinal pigment epithelial cell line with human herpesvirus 6 variant A
✍ Arao, Yujiro; Soushi, Sumi; Sato, Yuko; Moriishi, Eiko; Ando, Yasutaka; Yamada, 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 525 KB

A retinal pigment epithelial (RPE) cell line (K-1034) was examined for its susceptibility to human herpesvirus 6 variant A (HHV-6A). Exposure of K-1034 cells to HHV-6A induced the formation of multinucleated giant cells, which was suppressed by an inhibitor of viral DNA synthesis. In the giant cells

Human herpesvirus 6 infection of human e
Human herpesvirus 6 infection of human epidermal cell line: Pathogenesis of skin manifestations
✍ Dr. Tetsushi Yoshikawa; Fumi Goshima; Shiho Akimoto; Takao Ozaki; Takuya Iwasaki 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 185 KB

## Abstract In order to elucidate the pathogenesis of variant B human herpesvirus 6 (HHV‐6) infection in skin tissues, an A431 cell line was inoculated with variant B HHV‐6. HHV‐6 causes abortive infection in the A431 cells, because neither late antigen (OHV‐3 antigen) nor progeny virus is produced

Antibody reactivity with two strains of
Antibody reactivity with two strains of human herpesvirus-6 in Malaysians
✍ Dr. M. Yadav; S. Umamaheswari; D. Ablashi 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 373 KB

## Abstract A total of 234 sera from healthy Malaysians of diverse ethnic origins were tested for antibody to the Z29 and prototype GS strains of HHV‐6. The prevalence in the races ranged from 58 to 80% for the GS strain and 49 to 76% for the Z29 strain. The highest prevalence was in Malays with se