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Human erythrocyte catechol-o-methyltransferase: Correlation with lung and kidney activity

✍ Scribed by Richard M. Weinshilboum

Book ID
118938501
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
300 KB
Volume
22
Category
Article
ISSN
0024-3205

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Inhibition of human erythrocyte and gast
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The effect of increasing single oral doses of the novel catechol-O-methyltransferase (COMT) inhibitor, nitecapone, on enzyme activity in red cells (RBC) and gastroduodenal COMT activity has been studied in healthy male volunteers. A dose-dependent decrease in RBC COMT activity was seen in all cases

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The multistep assay of specific catechol-O-methyltransferase (COMT) activity in human erythrocytes was validated. Enzyme preparations from lysed erythrocytes were incubated with a substrate (3,4-dihydroxybenzoic acid) in the presence of Mg2+ and S-adenosylmethionine. The reaction products (vanillic