Ionic influence on human erythrocyte catechol-O-methyltransferase activity
✍ Scribed by Ylva Floderus; Jan Sääf; Svante B. Ross; Lennart Wetterberg
- Book ID
- 115775765
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 260 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0006-2952
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📜 SIMILAR VOLUMES
The effect of increasing single oral doses of the novel catechol-O-methyltransferase (COMT) inhibitor, nitecapone, on enzyme activity in red cells (RBC) and gastroduodenal COMT activity has been studied in healthy male volunteers. A dose-dependent decrease in RBC COMT activity was seen in all cases
The multistep assay of specific catechol-O-methyltransferase (COMT) activity in human erythrocytes was validated. Enzyme preparations from lysed erythrocytes were incubated with a substrate (3,4-dihydroxybenzoic acid) in the presence of Mg2+ and S-adenosylmethionine. The reaction products (vanillic