We report a new method for protecting nucleosides for hydrogen phosphonate RNA synthesis that gives very high yields for the purines. This method is based on our finding that, for adenosine and guanosine, silylation of a mixture of 2' and 3' H-phosphonates in the presence of DBU is highly selective
High yield protection of purine ribonucleosides for phosphoramidite RNA synthesis
β Scribed by Quanlai Song; Weimin Wang; Artur Fischer; Xiaohu Zhang; Barbara L. Gaffney; Roger A. Jones
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- French
- Weight
- 259 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0040-4039
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π SIMILAR VOLUMES
An efficient synthesis of protected ribonucleosides useful for phosphoramidite RNA synthesis is described. Di-t-butylsilylene group was employed for simultaneous protection of 3%-and 5%-hydroxyl functions of nucleoside. Subsequent silylation of free 2%-OH group followed by introduction of suitable p
The synthesis of atlyl-and allyloxycarbonyl (AOC)-protected RNA phosphoramidites is reporlexl. The use of allyl and AOC groups allows the chemistry of solid-phase RNA synthesis to be expanded to include base-labile modified nucleosides, such as acetylcytidine. The allyl-and AOCprotective chemistry c