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An efficient preparation of protected ribonucleosides for phosphoramidite RNA synthesis

✍ Scribed by Vladimir Serebryany; Leonid Beigelman

Publisher
Elsevier Science
Year
2002
Tongue
French
Weight
111 KB
Volume
43
Category
Article
ISSN
0040-4039

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✦ Synopsis

An efficient synthesis of protected ribonucleosides useful for phosphoramidite RNA synthesis is described. Di-t-butylsilylene group was employed for simultaneous protection of 3%-and 5%-hydroxyl functions of nucleoside. Subsequent silylation of free 2%-OH group followed by introduction of suitable protection on the base moiety, removal of cyclic silyl protection and tritylation of 5%-OH gave target compounds in 60-66% overall yield.

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