Recent progress in high-throughput enzyme assays includes new examples of fluorogenic and chromogenic substrates, fluorescence resonance energy transfer substrates, and applications of the pH and pM indicator methods. Recent developments of Horeau's pseudo-enantiomer derivatisation method to screen
High-throughput screening
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 308 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0952-7915
No coin nor oath required. For personal study only.
β¦ Synopsis
In Brier
Enhanced fluorescence microscopy
Spurred on by the development of new fluorochromes, fluorescence microscopy has diversified to fulfil a variety of research requirements in many medical and biological fields. With the BX Series, Olympus has introduced new refinements to provide a solution to the problems caused by the inhomogeneity of image brightness in fluorescence microscopy. The infinity-corrected UIS objectives are designed to eliminate optical aberrations and deliver high resolution and contrast. This is combined with improvements in the near ultraviolet transmission and vertical illuminator to provide consistently sharp images. The uniqt, e Y-shape frame of the BX series provides a stable support fi)r the attachment of accessories, such as CCD or other highly sensitive cameras. hnages can also be captured for documentation using the state-of-theart PM30 and PM20 Systems. The new FL Auto mode automatically measures and corrects exposure to precisely adjust for the exacting conditions of fluorescence photomicrography.
Circle number 1 on reader response card.
π SIMILAR VOLUMES
Directed evolution is becoming a widely used technique for modifying or enhancing protein performance. Ultimately, the success of directed protein evolution experiments hinges on the efficiency of the methods used to screen libraries for mutants with properties of interest. Although there is still a
To screen efficiently the millions of compounds that are synthesized using combinatorial and automated methods, dramatically improved assay technologies are currently needed. In 96-well microtiter plates, nonradioactive techniques (primarily fluorimetric) and cell-based functional methods have moved
A variety of assay technologies continue to be developed for high-throughput screening. These include cell-based assays, surrogate systems using microbial cells such as yeast and bacterial two-hybrid and three-hybrid systems, and systems to measure nucleic acid-protein and receptor-ligand interactio