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✦   LIBER   ✦

High-throughput screening of enzyme libraries

✍ Scribed by Mark Olsen; Brent Iverson; George Georgiou

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
757 KB
Volume
11
Category
Article
ISSN
0958-1669

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✦ Synopsis

Directed evolution is becoming a widely used technique for modifying or enhancing protein performance. Ultimately, the success of directed protein evolution experiments hinges on the efficiency of the methods used to screen libraries for mutants with properties of interest. Although there is still a paucity of general methods for enzyme library screening, in recent years a number of promising strategies have emerged and are increasingly being used to explore challenging issues in protein engineering.

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