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High-pressure liquid chromatography of peptides and proteins : VI. Rapid analysis of peptides by high-pressure liquid chromatography with hydrophobic ion-pairing of amino groups

✍ Scribed by W.S. Hancock; C.A. Bishop; L.J. Meyer; D.R.K. Harding; M.T.W. Hearn

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
498 KB
Volume
161
Category
Article
ISSN
1873-3778

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✦ Synopsis

This report describes the use of hydrophobic ion-pairing reagents in the rapid analysis of peptides by reversed-phase high-pressure liquid chromatography. It was found that combination of a hydrophobic anion such as hexanesulphonate with the c&ionic groups (R&H,) of a peptide resulted in a decreased polarity of the sample. This change in polarity resulted in an increased retention time on a FBondapakalkylphenyl column. In addition, the use of different ion-pairing reagents a!lowed dramatic changes in the selectivity of the reversed-phase system. This is demonstrated with peptides which range from tri-to heptapeptides using the following ion-pairing reagents : phosphoric acid, sodium hexanesulphonate and sodium dodecyl sulphate.

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