High-pressure liquid chromatography of modified purine mononucleotides and nucleosides

✍ Scribed by K.-W. Stahl; E. Schlimme; D. Bojanowski

• Book ID: 104145037
• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 568 KB
• Volume: 83
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)97056-0

✦ Synopsis

The useful application of high-pressure liquid chromatography in biochemical research with adenine nucleotide analogues is demonstrated by using a glass-walled separating column at inlet pressures between 100 and 200 atm. Three different aspects are highlighted :

(1) purity control of the adenine nucleotide analogues;

(2) direct reaction monitoring by high-pressure liquid chromatography;

(3) high-pressure liquid chromatography data and mononucleotide conformation.