✦ LIBER ✦

Extraction and measurement of myocardial nucleotides, nucleosides, and purine bases by high-performance liquid chromatography

✍ Scribed by Donald F. Hammer; Donald V. Unverferth; Robert E. Kelley; Paula A. Harvan; Ruth A. Altschuld

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 436 KB
Volume: 169
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(88)90288-6

No coin nor oath required. For personal study only.

✦ Synopsis

Nucleotides, nucleosides, and purine bases were extracted from human endomyocardial biopsies, freeze-clamped rat hearts, and porcine coronary sinus plasma. Perchloric acid extracts were neutralized with Freon-trioctylamine and analyzed at 250 nm by reverse-phase ion-pairing high-performance liquid chromatography. To achieve the sensitivity necessary for analyzing small (1-3 mg wet wt) tissue samples, a small-bore, 2.1-mm-internal-diameter, C18, 5-micron reverse-phase column and a flow rate of 0.2 ml/min were used. All of the myocardial nucleotides and AMP degradation products were resolved in a total separation time of 27 min with 30 mM KH2PO4, 7.5 mM tetrabutylammonium phosphate buffers, and binary pH and acetonitrile gradients.

📜 SIMILAR VOLUMES

Extraction and Assay of Creatine Phospha

Extraction and Assay of Creatine Phosphate, Purine, and Pyridine Nucleotides in Cardiac Tissue by Reversed-Phase High-Performance Liquid Chromatography

✍ P. Bernocchi; C. Ceconi; A. Cargnoni; P. Pedersini; S. Curello; R. Ferrari 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 379 KB

Simultaneous Determination of Creatine C

Simultaneous Determination of Creatine Compounds and Adenine Nucleotides in Myocardial Tissue by High-Performance Liquid Chromatography

✍ T. Teerlink; M. Hennekes; J. Bussemaker; J. Groeneveld 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 475 KB

Measurement of liver adenine nucleotides

Measurement of liver adenine nucleotides and S-adenosyl amino acids by one-step high-performance liquid chromatography

✍ Henriette Gourdeau; Robert Lavoie; John H. Grose; Luc Bélanger 📂 Article 📅 1986 🏛 Elsevier Science 🌐 English ⚖ 262 KB

A reverse-phase isocratic HPLC method is described for direct simultaneous assay of ATP, ADP. AMP, S-adenosylmethionine, S-adenosylhomocysteine, S-adenosylethionine, and other adenine derivatives in liver microbiopsies. The procedure was tested in conditions which alter the hepatic content of adenin

Separation of nucleosides and nucleotide

Separation of nucleosides and nucleotides by reversed-phase high-performance liquid chromatography with volatile buffers allowing sample recovery

✍ Charlotte Yu Ip; Daniel Ha; Paul W. Morris; Mark L. Puttemans; Duane L. Venton 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 479 KB

Reversed-phase high-performance liquid chromatography using a C,8 column with volatile buffers as the eluant was applied to the separation of a number of nucleosides and nucleotides. Groups of seven nucleosides and five nucleoside monophosphates were separated isocratically employing 0.1 M trimethyl

The measurement of nucleoside deaminases

The measurement of nucleoside deaminases by high performance liquid chromatography and their use in clinical chemistry

✍ R. A. Sherwood 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 415 KB

Measurement of serum unconjugated estrio

Measurement of serum unconjugated estriol and estradiol by high-performance liquid chromatography

✍ Zenjiro Kondo; Tsunehisa Makino; Rihachi Lizuka 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 327 KB

## Abstract Measurement of Estriol (E~3~) and estradiol (E~2~) within 22 min by high‐performance liquid chromatography (HPLC) was achieved in this study, and the values were compared with those of a radioimmunoassay (RIA). A totally computerized HPLC method was developed for measuring unconjugated