High-performance liquid chromatography analysis of purine nucleosides in human gingival crevicular fluid
β Scribed by E.G.E. Jahngen; M. Brecx; E.F. Rossomando
- Book ID
- 115711961
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 454 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0003-9969
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π SIMILAR VOLUMES
A high-performance liquid chromatographic method was developed for the determination of plasma purine nucleoside phosphorylase activity. In this method, the reaction mixture consisted of \(15 \mu\) l of plasma and \(285 \mu \mathrm{l}\) of \(50 \mathrm{mM}\) phosphate buffer ( \(\mathrm{pH} 7.4\) )
A high-pressure liquid chromatography method has been developed for the analysis in urinary calculi of six purines: uric acid, 2,8-dihydroxyadenine, xanthine, hypoxanthine, allopurinol, and oxypurinol. Separation was conducted isocratically on a reversed-phase column, using 50 mM phosphate buffer (p