High-pressure liquid chromatography of carcinogen-nucleoside conjugates: Separation of 7,12-dimethylbenzanthracene derivatives

✍ Scribed by Alan M. Jeffrey; Steven H. Blobstein; I.Bernard Weinstein; Ronald G. Harvey

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 464 KB
• Volume: 73
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90183-4

✦ Synopsis

Conditions for obtaining rapid, high resolution separations of nucleic acid derivatives of 7,12-dimethylbenzanthracene using reverse phase high-pressure liquid chromatography are described. Direct reaction of dimethylbenzanthracene-5,6-oxide with poly(G) or poly(A) yields four and two major dimethylbenzanthracene-nucleoside conjugates. respectively. Incubation of [3H]dimethylbenzanthracene with poly( G) in a microsomal system leads to the formation of numerous guanosine derivatives. most of which differ from the 5.6oxide products. The high sensitivity and resolution obtained in these studies demonstrate that high-pressure liquid chromatography will be a valuable technique for identification and characterization of other carcinogen-nucleic acid complexes.