✦ LIBER ✦

High-level production of recombinant immunoglobulins in mammalian cells

✍ Scribed by Mitchell E. Reff

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 367 KB
Volume: 4
Category: Article
ISSN: 0958-1669
DOI: 10.1016/0958-1669(93)90079-c

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✦ Synopsis

Successful high-level expression of recombinant immunogtobulins has now been reported in several different types of mammalian cells. This has been achieved using random integration of immunoglobulin genes co-linked with a drug-resistance gene into cellular DNA. After gene integration, increases in immunoglobulin-gene expression are obtained by selection for increases in expression of the drug-resistance marker. Expression levels attained by this method are several fold higher than those produced by a good hybridoma. Soon, site-specific homologous recombination may replace random integration as the favored methodology for initial expression of all proteins in mammalian cells, including immunoglobulins.

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