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Production of target-specific recombinant human polyclonal antibodies in mammalian cells

✍ Scribed by Finn C. Wiberg; Søren K. Rasmussen; Torben P. Frandsen; Lone K. Rasmussen; Kaja Tengbjerg; Vincent W. Coljee; Jacqueline Sharon; Chiou-Ying Yang; Søren Bregenholt; Lars S. Nielsen; John S. Haurum; Anne B. Tolstrup

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
494 KB
Volume
94
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

We describe the expression and consistent production of a first target‐specific recombinant human polyclonal antibody. An anti‐Rhesus D recombinant polyclonal antibody, Sym001, comprised of 25 unique human IgG1 antibodies, was produced by the novel Sympress expression technology. This strategy is based on site‐specific integration of antibody genes in CHO cells, using the FRT/Flp‐In recombinase system. This allows integration of the expression construct at the same genomic site in the host cells, thereby reducing genomic position effects. Different bioreactor batches of Sym001 displayed highly consistent manufacturing yield, antibody composition, binding potency, and functional activity. The results demonstrate that diverse recombinant human polyclonal antibody compositions can be reproducibly generated under conditions directly applicable to industrial manufacturing settings and present a first recombinant polyclonal antibody which could be used for treatment of hemolytic disease of the newborn and/or idiopathic thrombocytopenic purpura. © 2006 Wiley Periodicals, Inc.

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