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Kinetics of recombinant immunoglobulin production by mammalian cells in continuous culture

✍ Scribed by David K. Robinson; Klaus W. Memmert

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
430 KB
Volume
38
Category
Article
ISSN
0006-3592

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✦ Synopsis

A clonal derivative of a transfectant of the SP2/0 myeloma cell line producing a chimeric monoclonal antibody was maintained in steady-state, continuous culture at dilution rates ranging from 0.21 to 1.04 day-'. The steady-state values for nonviable and total cell concentrations increased as the dilution rate decreased, while the viable cell concentration was roughly independent of the dilution rate. At steady state, the specific growth rate increased and the specific death rate decreased as the dilution rate increased. The maximum specific growth rate was 1.15 day-'. Antibody production was growth associated and the specific rate of antibody production increased linearly as the specific growth rate increased.

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