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High level production of recombinant invertase inHansenula polymorpha

โœ Scribed by R. E. Narciandi; L. Rodriguez; E. Rodriguez; R. Diaz; J. Delgado; L. Herrera

Publisher
Springer Netherlands
Year
1995
Tongue
English
Weight
237 KB
Volume
17
Category
Article
ISSN
0141-5492

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โœฆ Synopsis

The WC 2 gene from Saccharomyces cerevisiae coding for the enzyme lnvertase was cloned in Hansenula polymorpha under the control of the alcohol-oxidase (AOX) promoter of Pichia pastoris. The culture conditions for the Inve,$ase production using a fed-batch culture were studied. More than I,5 x 10 U/ml of lnvertase (1 g/L) were found to be secreted to cellular periplasmic space. The scale up to 50 L of the fermentative process was done.

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