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High-Level Expression and Novel Purification Strategy of Recombinant Thanatin Analog inEscherichia coli

✍ Scribed by G.-Q. Wu; L.-X. Li; J.-X. Ding; L.-Z. Wen; Z.-L. Shen


Publisher
Springer
Year
2008
Tongue
English
Weight
288 KB
Volume
57
Category
Article
ISSN
0343-8651

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The high-level expression plasmid for streptokinase, pSK100, has been constructed. It contains a tac promoter, an ompA signal sequence, a streptokinase structural gene(skc) and a rrnBT1T2 transcription terminator. E. coli JM109 carrying pSK100 produced about 5,000IU of streptokinase per 1 ml of LB-a