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High level expression and purification of antimicrobial human cathelicidin LL-37 inEscherichia coli

✍ Scribed by Ján Krahulec; Marcela Hyršová; Stanislav Pepeliaev; Jana Jílková; Zbyněk Černý; Jana Machálková

Publisher
Springer
Year
2010
Tongue
English
Weight
363 KB
Volume
88
Category
Article
ISSN
1432-0614

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The high-level expression plasmid for streptokinase, pSK100, has been constructed. It contains a tac promoter, an ompA signal sequence, a streptokinase structural gene(skc) and a rrnBT1T2 transcription terminator. E. coli JM109 carrying pSK100 produced about 5,000IU of streptokinase per 1 ml of LB-a