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Hda, a novel DnaA-related protein, regulates the replication cycle in Escherichia coli

✍ Scribed by Kato, J.-i.

Book ID
111748068
Publisher
Nature Publishing Group
Year
2001
Tongue
English
Weight
311 KB
Volume
20
Category
Article
ISSN
0261-4189

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Flow cytometry was used to study initiation of DNA replication in Escherichia coli K12 after induced expression of a plasmid-borne dnaA+ gene. When the dnaA gene was induced from either the plac or the lambda pL promoter initiation was stimulated, as evidenced by an increase in the number of origins